Invitro assays
In Vitro Toxicity Assays:
- Cytotoxicity- MTT, SRB, Trypan Blue, LDH, SDH Assays
- Cell apoptosis- Caspase 3, Caspase 9, BCL-BAX, Hoechst/ PI assay, EtBr/ AO staining assay
- Organ-Specific Toxicity –Renal, Hepato, cardiac, Neuro, Immuno Toxicity, etc.
In vitro Bioassays:
- Antimicrobial activity (Antibacterial, Antifungal, Antiprotozoal, Antialgal, Anticandidal)
- Anticancer activity (Cytotoxicity, DNA fragmentation, Apoptotic Protein, Clonogenic assay)
- Antioxidant activity (DPPH / ABTS scavenging, FRAP, TRAP, Reducing power, etc.)
- Anti-inflammatory, Anti-hyperlipidemia
- Enzyme assays (Qualitative/quantitative, Enzyme screenings, Enzyme kinetics,)
- Wound healing study
- Anti-coagulant activity
- Anti-thrombotic activity
- Membrane stabilization test
- Heat-induced hemolysis
- Cytoprotective assay
- endocrine disruptor screening
Liver Health
- liver injury
- fibrogenesis or fibrinolysis
- NASH assay
- Cirrhosis models
- Cystinosis model
- Hepatoprotection against toxins (tBHP, Ethanol, organ-pesticides etc)
- Enzyme induction studies using HepG2
Inflammation
- Cytokine/chemokine production assay for inflammatory response/inhibition assessment
- Stimuli: LPS, PMA, PHA, Con A CD3/CD28, etc.
- T cell and B cell proliferation in response to specific antigens or mitogens
- Trans-well chemotaxis assay with eosinophils, neutrophils, monocytes, B cells, T cells, etc.
- Inflammation related cell signalling pathway study
Diabetics and Obesity Model
- Adipolysis and adipogenesis using 3T3-L1, C3H10T1/2 cell lines
- Glucose uptake studies
Cell line based functional assay:
- Glycolytic pathway
- Amino acid synthesis pathway
- Nucleic acid synthesis pathway
- Cell signalling pathway
- Neurotransmission evaluation
