Invitro assays
In vitro Bioassays (non-cell line based):
- Antimicrobial activity (Antibacterial, Antifungal)
- Antioxidant activity (DPPH / ABTS scavenging, FRAP, TRAP, Reducing power, etc.)
- Anti-inflammatory, Anti-hyperlipidemia
- Enzyme assays (Qualitative/quantitative, Enzyme screenings, Enzyme kinetics,)
- Anti-coagulant activity
- Anti-thrombotic activity
- Membrane stabilization test
- Heat-induced hemolysis
In Vitro Assays (cell line based):
- Cytotoxicity- MTT, Trypan Blue Assays
- Anticancer activity (Cytotoxicity, DNA fragmentation, Apoptotic Protein, Clonogenic assay)
- Cell apoptosis- Caspase 3, Caspase 9, BCL-BAX, Hoechst/ PI assay, EtBr/ AO staining assay
- Anti-oxidant (ROS) assay
- Organ-Specific Toxicity –Renal, Hepato, cardiac, Neuro, Immuno Toxicity, etc.
- Wound healing study
- Adipolysis and adipogenesis using 3T3-L1, C3H10T1/2 cell lines
- Glucose uptake studies
- Neurodegeneration assay
- Cytoprotective assay
- And all cell line based efficacy studies
